Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14948] to Thrombopoietin
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
Reacts with: Human, Recombinant fragment

Product name

Anti-Thrombopoietin antibody [EPR14948]
See all Thrombopoietin primary antibodies
Description

Rabbit monoclonal [EPR14948] to Thrombopoietin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver; Thrombopoietin recombinant protein; Human liver and bone marrow tissues; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14948
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

Note: Cytoplasm staining on human bone marrow tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Thrombopoietin with purified ab196026 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Cytoplasm staining on Jurkat cell line was observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

Note: Cytoplasm staining on human liver tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/5000 dilution + Thrombopoietin recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa
Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)

Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/1000 dilution + Human fetal liver at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 38 kDa
Anti-Thrombopoietin antibody [EPR14948] (ab196026)

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