Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)
![Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315952-anti-angiotensinogen-antibody-epr22199-203-western-blot.jpg "Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22199-203] to Angiotensinogen
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Angiotensinogen antibody [EPR22199-203]
See all Angiotensinogen primary antibodies -
Description
Rabbit monoclonal [EPR22199-203] to Angiotensinogen -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver, kidney, heart, brain and lung tissue lysate. IHC-P: Mouse kidney and testis tissue. IP: Mouse liver tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22199-203 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)All lanes : Anti-Angiotensinogen antibody [EPR22199-203] (ab229005) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Exposure time: 114 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315954-anti-angiotensinogen-antibody-epr22199-203-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID: 7648615) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315955-anti-angiotensinogen-antibody-epr22199-203-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID: 20361351) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315953-anti-angiotensinogen-antibody-epr22199-203-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: Mouse liver lysate (10 μg) (Input).
Lane 2: ab29005 IP in mouse liver lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).Blocking/D ilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds. -
![Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-5-benefits-of-recombinant-antibodies.png)
Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)
