Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22199-203] to Angiotensinogen
Suitable for: WB, IHC-P, IP
Reacts with: Mouse

Product name

Anti-Angiotensinogen antibody [EPR22199-203]
See all Angiotensinogen primary antibodies
Description

Rabbit monoclonal [EPR22199-203] to Angiotensinogen
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse liver, kidney, heart, brain and lung tissue lysate. IHC-P: Mouse kidney and testis tissue. IP: Mouse liver tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22199-203
Isotype

IgG
Research areas

Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

All lanes : Anti-Angiotensinogen antibody [EPR22199-203] (ab229005) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa

Exposure time: 114 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID: 7648615) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID: 20361351) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: Mouse liver lysate (10 μg) (Input).
Lane 2: ab29005 IP in mouse liver lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).

Blocking/D ilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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