Unpurified ab181086 staining Parvalbumin in mouse cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).

The section was incubated with ab181086 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

ab181086 staining Parvalbumin in rat cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/250). ab150077 an AlexaFluor^®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on purkinje cells and neuron cells of rat cerebellum.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unpurified ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).

Unpurified ab181086 staining Parvalbumin in rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on Purkinje cells and neurons of the molecular layer in rat cerebellum (PMID: 22561329).

Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on mouse skeletal muscle (PMID: 1837548).

Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

Positive staining on skeletal muscles (PMID: 7604022).

ab181086 (purified) at 1:20 dilution (1µg) immunoprecipitating Parvalbumin in Human cerebellum lysate.

Lane 1: Human cerebellum lysate 10μg
Lane 2 (+): ab181086 & Human cerebellum lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181086 in Human cerebellum lysate
For western blotting, VeriBlot for IP Detection reagent (HRP) (ab131366) was used at 1:5000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.

All lanes : Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution ((unpurified))

Lane 1 : Mouse skeletal muscle lysate prepared using RIPA lysis method
Lane 2 : Rat skeletal muscle lysate prepared using RIPA lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa
Observed band size: 12 kDa


Exposure time: 75 seconds

All lanes : Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution ((unpurified))

Lane 1 : Mouse cerebellum lysate prepared using RIPA lysis method
Lane 2 : Mouse cerebellum lysate prepared using 1% SDS hot lysis method
Lane 3 : Rat cerebellum lysate prepared using 1% SDS hot lysis method
Lane 4 : Human brain lysate prepared using RIPA lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 12 kDa
Observed band size: 12 kDa


Exposure time: 3 minutes

The rat cerebellum lysate in lane 3 is prepared by 1%SDS hot lysis method. This antibody can work in RIPA and 1% SDS hot lysates in WB.

For Lysate preparation protocol, please refer to the protocol book in the protocol section.

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Parvalbumin with ab181086 at a 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human chromophobe carcinoma tissue labeling Parvalbumin with unpurified ab181086 at a 1/500 dilution.

Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/10000 dilution ((unpurified)) + Human cerebellum tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Developed using the ECL technique.

Predicted band size: 12 kDa

Western blot analysis on immunoprecipitation pellet from Human cerebellum tissue lysate labeling Parvalbumin with unpurified ab181086 at 1/40 dilution.