Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
![Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)](https://www.abcam.com/ps/products/255/ab255926/Images/ab255926-3-anti-cd22-antibody-4kb128-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4KB128] to CD22 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CD22 antibody [4KB128] - BSA and Azide free
See all CD22 primary antibodies -
Description
Mouse monoclonal [4KB128] to CD22 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: Raji cells; human PBMC cells. Flow Cyt: Human PBMCs.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255926 is the carrier-free version of ab254171. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4KB128 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CD22 with ab254171 at 2.692 μg/mL
(Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with mouse IgG and ab254171, then stained with anti-CD19 conjugated to Alexa Fluor® 647.Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)](https://www.abcam.com/ps/products/255/ab255926/Images/ab255926-2-anti-cd22-antibody-4kb128-immunocytochemistry-human-pbmc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC cells labelling CD22 with ab254171 at 13.46 μg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). Confocal image showing cytoplamic and membranous staining in subsets of human PBMCs. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)](https://www.abcam.com/ps/products/255/ab255926/Images/ab255926-1-anti-cd22-antibody-4kb128-immunocytochemistry-raji.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Raji cells labelling CD22 with ab254171 at 13.46 μg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor®488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic and membranous staining in Raji cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).
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![Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)](https://www.abcam.com/ps/products/255/ab255926/Images/ab255926-4-benefits-of-recombinant-antibodies.png)
Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)