Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4KB128] to CD22 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD22 antibody [4KB128] - BSA and Azide free
    See all CD22 primary antibodies

  • Description

    Mouse monoclonal [4KB128] to CD22 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: Raji cells; human PBMC cells. Flow Cyt: Human PBMCs.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255926 is the carrier-free version of ab254171. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
    Flow Cytometry - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)

    Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CD22 with ab254171 at 2.692 μg/mL
    (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with mouse IgG and ab254171, then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
    Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC cells labelling CD22 with ab254171 at 13.46 μg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). Confocal image showing cytoplamic and membranous staining in subsets of human PBMCs. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
    Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Raji cells labelling CD22 with ab254171 at 13.46 μg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor®488) antibody at 1/1000  dilution (Green). Confocal image showing cytoplamic and membranous staining in Raji cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254171).

  • Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)
    Anti-CD22 antibody [4KB128] - BSA and Azide free (ab255926)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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