Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Mouse CD28 protein (Fc Chimera)
    See all CD28 proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P31041

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      NKILVKQSPLLVVDSNEVSLSCRYSYNLLAKEFRASLYKGVNSDVEVCVG NGNFTYQPQFRSNAEFNCDGDFDNETVTFRLWNLHVNHTDIYFCKIEFMY PPPYLDNERSNGTIIHIKEKHLCHTQSSPK

    • Predicted molecular weight

      42 kDa including tags

    • Amino acids

      20 to 149

    • Additional sequence information

      Fused with a Human IgG1 Fc tag at the C-terminus (NP_031668.3).

    • Specifications

    Our Abpromise guarantee covers the use of ab207114 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

      pH: 7.4
      Constituents: 0.75% Glycine, 0.61% Tris, 5% Trehalose, Sodium chloride, L-Arginine

      Lyophilized from 0.22 µm filtered solution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • CD 28
      • CD28
      • CD28 antigen
      • CD28 molecule
      • CD28_HUMAN
      • MGC138290
      • T cell antigen CD28
      • T cell specific surface glycoprotein
      • T cell specific surface glycoprotein CD28
      • T-cell-specific surface glycoprotein CD28
      • TP44
      see all

    • Function

      Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

    • Tissue specificity

      Expressed in T-cells and plasma cells, but not in less mature B-cells.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

Images

  • SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)
    SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)

    DTT-reduced SDS-PAGE analysis of ab207114 stained overnight with Coomassie Blue.

    DTT-reduced protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab207114? Please let us know so that we can cite the reference in this datasheet.

ab207114 has not yet been referenced specifically in any publications.

Preparation and Storage

  • CD 28
  • CD28
  • CD28 antigen

Images

  • SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)
    SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)

    DTT-reduced SDS-PAGE analysis of ab207114 stained overnight with Coomassie Blue.

    DTT-reduced protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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