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Immunology Adaptive Immunity T Cells CD

CF405M Anti-CD19 antibody [A3-B1] (ab117733)

CF405M Anti-CD19 antibody [A3-B1] (ab117733)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • CF405M Mouse monoclonal [A3-B1] to CD19
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: CF405M. Ex: 408nm, Em: 452nm
  • Isotype: IgG2a

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Overview

  • Product name

    CF405M Anti-CD19 antibody [A3-B1]
    See all CD19 primary antibodies
  • Description

    CF405M Mouse monoclonal [A3-B1] to CD19
  • Host species

    Mouse
  • Conjugation

    CF405M. Ex: 408nm, Em: 452nm
  • Specificity

    Directed against the CD19, which is expressed on human B lymphocytes. ab117733 is B lineage-specific and reacts with early B-cell precursors, pre-pre-B-cells, pre-B- cells, B-cells, intermediate B-cells, mature B-cells and some plasmacytoid cells. Plasma cells were found to be negative. It does not react with other haemopoïetic cells. Also reacts with pre-B-cell- lines, B lymphoblastoid cell-lines and Burkitt cell- lines, and with 50% of myeloma cell-lines. Virtually all non T-ALL, B-CLL and B-cell lymphomas were found to be positive, myeloma cells were found to be negative.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    CD19 antigen which is expressed on human B lymphocytes.

  • Positive control

    • Normal Human peripheral blood
  • General notes

    CD19 cluster expressed from the earliest stages of B-progenitor development and on all peripheral B cells including germinal centre B cells, all B cell lines tested and B cell leukaemias tested. The antigen is lost on B cell maturation to plasma cells.

    Fluorochrome: CF405M (Abs/Em Max: 408/450 nm) CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservatives: 0.09% Sodium azide, 0.028% Proclin 300
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    CD19 cluster expressed from the earliest stages of B-progenitor development and on all peripheral B cells including germinal centre B cells, all B cell lines tested and B cell leukaemias tested. The antigen is lost on B cell maturation to plasma cells.
  • Clonality

    Monoclonal
  • Clone number

    A3-B1
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Images

  • Flow Cytometry - CF405M Anti-CD19 antibody [A3-B1] (ab117733)
    Flow Cytometry - CF405M Anti-CD19 antibody [A3-B1] (ab117733)
    Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).
  • Flow Cytometry - CF405M Anti-CD19 antibody [A3-B1] (ab117733)
    Flow Cytometry - CF405M Anti-CD19 antibody [A3-B1] (ab117733)
    Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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