Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD19 antibody [SP110] - C-terminal (ab225717)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP110] to CD19 - C-terminal
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD19 antibody [SP110] - C-terminal
    See all CD19 primary antibodies

  • Description

    Rabbit monoclonal [SP110] to CD19 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Ramos and Raji cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-CD19 antibody [SP110] - C-terminal (ab225717)
    Flow Cytometry - Anti-CD19 antibody [SP110] - C-terminal (ab225717)

    Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab225717 at 1/40 dilution (11.725 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP110] - C-terminal (ab225717)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP110] - C-terminal (ab225717)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using ab225717 at 1/200 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD19 antibody [SP110] - C-terminal (ab225717)
    Flow Cytometry - Anti-CD19 antibody [SP110] - C-terminal (ab225717)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD19 with ab225717 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue)

  • Anti-CD19 antibody [SP110] - C-terminal (ab225717)
    Anti-CD19 antibody [SP110] - C-terminal (ab225717)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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