Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23174-145] to CD19 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-Fr, WB, IHC-P, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD19 antibody [EPR23174-145] - BSA and Azide free
    See all CD19 primary antibodies

  • Description

    Rabbit monoclonal [EPR23174-145] to CD19 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-Fr, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen tissue lysate. WEHI-231 and A20 whole cell lysate. IHC-P: Mouse spleen tissue. ICC/IF: Mouse peripheral blood mononuclear cells. Flow Cyt: Mouse peripheral blood mononuclear cells. IP: Mouse spleen lysate. IHC-Fr: Mouse spleen tiissue.

  • General notes

    ab267394 is the carrier-free version of ab245235.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Immunohistochemistry (Frozen sections) - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

    This data was developed using ab267394 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized perfusion fixed frozen Mouse spleen tissue labeling CD19 with ab245235 at 1/250 (1.82 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse spleen. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD19 with ab245235 at 1/1000 dilution (0.446 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on mouse spleen (PMID: 18648532). The section was incubated with ab245235 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245235).

  • Immunoprecipitation - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Immunoprecipitation - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

    CD19 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab245235 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245235 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen lysate 10µg

    Lane 2: ab245235 IP in mouse spleen lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245235 in mouse spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245235).

  • Flow Cytometry - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Flow Cytometry - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

    Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labeling CD19 with ab245235 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab245235 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. CD19 and CD3 are mutually exclusive expressed in mouse PBMC.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245235).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labeling CD19 with ab245235 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subsets of mouse PBMCs. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245235).

  • Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)
    Anti-CD19 antibody [EPR23174-145] - BSA and Azide free (ab267394)

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