Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP573-4] to Syk (phospho Y323) - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free
    See all Syk primary antibodies

  • Description

    Rabbit monoclonal [EP573-4] to Syk (phospho Y323) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects Syk phosphorylated at Tyrosine 323.

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, Dot blot, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lymph node tissue.

  • General notes

    ab62338 is the carrier-free version of ab169865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab62338 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)

    Immunocytochemistry/Immunofluorescence analysis of Ramos+/-pervanadate(1mM, 30min), Ramos + pervanadate(1mM, 30min) + LP labelling Syk (phospho Y323) with ab62338 at a dilution of 1:1000 dilution (2.53ug/ml). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) (1:1000) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62338).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    This data was developed using the same antibody clone in a different buffer formulation (ab62338).
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue sections labeling Syk with purified ab62338 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
    Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
  • Flow Cytometry - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    Flow Cytometry - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)

    Flow Cytometry analysis of U937 (human monocyte histiocytic lymphoma) treated (red)/untreated (green) with 1mM pervanadate for 30 minutes with purified ab62338 at 1/2500 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62338).

  • Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)

    Dot blot analysis of Sky (pY323) peptide (Lane 1), Syk (unmodified) peptide (Lane 2), labelling Syk (pY323) with ab62338 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62338).

  • Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)
    Anti-Syk (phospho Y323) antibody [EP573-4] - BSA and Azide free (ab169865)

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