Lanes 1-2 : Anti-Syk antibody [SYK-01] (ab3993) at 2 µg/ml
Lanes 3-4 : anti-Human Cytokeratin 18


Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : RBL (rat basophilic leukemia cell line) cell lysate
Lanes 3-4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : negative control

Performed under non-reducing conditions.

Immunocytochemistry analysis of Syk in human HeLa (human epithelial cell line from cervix adenocarcinoma) cell line using ab3993 (green) at 5 µg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry analysis of Syk in human primary fibroblasts using ab3993 (green) at 5 μg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Overlay histogram showing Ramos (Human Burkitt's lymphoma cell line) cells stained with ab3993 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3993, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.