Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP573Y] to Syk - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Syk antibody [EP573Y] - BSA and Azide free
    See all Syk primary antibodies

  • Description

    Rabbit monoclonal [EP573Y] to Syk - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, Daudi, WEHI-231 and Raji cell lysates. Human, mouse and rat bone marrow lysates. IHC-P: Human spleen. ICC/IF: Raji and Daudi cells. Flow Cyt: Raji and K562 cells.

  • General notes

    Ab190176 is the carrier-free version of ab40781. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab190176 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Western blot - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    All lanes : Anti-Syk antibody [EP573Y] (ab40781) at 1/1000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
    Lane 3 : WEHI-231 (Mouse B cell lymphoma B lymphocyte ) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

    Immunohistochemistry of paraffin embedded Human spleen tissue section labelling Syk with ab40781 at 1:5000 dilution (0.44 μg/ml).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use ImmunoHistoProbe one step HRP Polymer (ready to use) was used as a secondary antibody at 1:0 dilution. PBS instead of primary antibody was used for negative control. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

    Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Syk with Purified ab40781 at 1:150 (5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Flow Cytometry - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Flow Cytometry - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte)  cells labeling Syk with Purified ab40781 at 1:70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Western blot - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Western blot - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Anti-Syk antibody [EP573Y] (ab40781) at 1/1000 dilution (Purified) + Human bone marrow lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 72 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

    Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) labelling Syk with ab40781 at a dilution of 1:100 dilution (7µg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) (1:1000 dilution (2 µg/ml)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5µg/ml). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Flow Cytometry - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Flow Cytometry - Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

    Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling Syk with unpurified ab40781 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40781).

  • Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)
    Anti-Syk antibody [EP573Y] - BSA and Azide free (ab190176)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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