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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-Syk (phospho Y525) antibody [EP575(2)Y]
    See all Syk primary antibodies
  • Description

    Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525)
  • Host species

    Rabbit
  • Specificity

    ab62350 recognises Syk phosphorylated at Tyrosine 525
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surround Tyrosine 525 of Human Syk

  • Positive control

    • U937 cell lysates treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP575(2)Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • Mast Cells

Images

  • Dot Blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
    Dot Blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: Syk(pY525) phospho peptide

    Lane 2 sample: Syk non-phospho peptide

    Exposure time: 3 minutes

  • Western blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
    Western blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
    All lanes : Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350) at 1/10000 dilution

    Lane 1 : untreated U937 cell lysates
    Lane 2 : U937 cell lysates treated with pervanadate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti rabbit HRP at 1/2000 dilution

    Predicted band size: 72 kDa
    Observed band size: 75,80 kDa
    why is the actual band size different from the predicted?



    Beta tubulin shows equal lysate loading amount.
  • Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
    Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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