Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Syk (phospho Y525) antibody [EP575(2)Y]
See all Syk primary antibodies -
Description
Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525) -
Host species
Rabbit -
Specificity
ab62350 recognises Syk phosphorylated at Tyrosine 525 -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
A phospho specific peptide corresponding to residues surround Tyrosine 525 of Human Syk
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Positive control
- U937 cell lysates treated with pervanadate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP575(2)Y -
Isotype
IgG -
Research areas
Images
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: Syk(pY525) phospho peptide
Lane 2 sample: Syk non-phospho peptide
Exposure time: 3 minutes
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All lanes : Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350) at 1/10000 dilution
Lane 1 : untreated U937 cell lysates
Lane 2 : U937 cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 75,80 kDa why is the actual band size different from the predicted?
Beta tubulin shows equal lysate loading amount. -