Anti-Syk antibody (ab155187)
Key features and details
- Rabbit polyclonal to Syk
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Syk antibody
See all Syk primary antibodies -
Description
Rabbit polyclonal to Syk -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within internal sequence amino acids 148-483 of Human Syk (UniProt P43405).
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Positive control
- WB: BMDM, Raji, A431, mouse spleen tissue; IHC-P: Human cervical cancer, endometrium; rat spleen tissue; IF: A431 whole cell lysate; IP: A431 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Syk antibody (ab155187) at 1/1000 dilution
Lane 1 : BMDM (Mouse bone marrow-derived macrophage) whole cell lysate
Lane 2 : Syk null cell
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunofluorescence analysis of methanol-fixed A431 (human epidermoid carcinoma cell line) cells labeling Syk with ab155187 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Immunoprecipitation analysis of SYK protein from A431 (human epidermoid carcinoma cell line) whole cell lysates.
IP samples:
1: A431 whole cell lysates;
2: Control with IgG;
3: Immunoprecipitate of Syk protein by 5 μg of ab155187.
Western blot analysis was performed using ab155187, followed by anti-Rabbit IgG antibody.
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Anti-Syk antibody (ab155187) at 1/2000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-Syk antibody (ab155187) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Anti-Syk antibody (ab155187) at 1/1000 dilution + Mouse spleen tissue at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa7.5% SDS-PAGE