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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-Syk antibody (ab155187)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Syk antibody (ab155187)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Syk
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Syk antibody
    See all Syk primary antibodies
  • Description

    Rabbit polyclonal to Syk
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 148-483 of Human Syk (UniProt P43405).

  • Positive control

    • WB: BMDM, Raji, A431, mouse spleen tissue; IHC-P: Human cervical cancer, endometrium; rat spleen tissue; IF: A431 whole cell lysate; IP: A431 whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • Mast Cells

Images

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    All lanes : Anti-Syk antibody (ab155187) at 1/1000 dilution

    Lane 1 : BMDM (Mouse bone marrow-derived macrophage) whole cell lysate
    Lane 2 : Syk null cell

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody (ab155187)
    Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody (ab155187)

    Immunofluorescence analysis of methanol-fixed A431 (human epidermoid carcinoma cell line) cells labeling Syk with ab155187 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunoprecipitation - Anti-Syk antibody (ab155187)
    Immunoprecipitation - Anti-Syk antibody (ab155187)

    Immunoprecipitation analysis of SYK protein from A431 (human epidermoid carcinoma cell line) whole cell lysates.

    IP samples:

    1: A431 whole cell lysates;

    2: Control with IgG;

    3: Immunoprecipitate of Syk protein by 5 μg of ab155187.

    Western blot analysis was performed using ab155187, followed by anti-Rabbit IgG antibody.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/2000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/1000 dilution + Mouse spleen tissue at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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