Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP573-4] to Syk (phospho Y323)
Suitable for: Flow Cyt, Dot blot, WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Syk (phospho Y323) antibody [EP573-4]
See all Syk primary antibodies
Description

Rabbit monoclonal [EP573-4] to Syk (phospho Y323)
Host species

Rabbit
Specificity

This antibody detects Syk phosphorylated at Tyrosine 323.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Tyrosine 323 of human Syk
Positive control
- WB: U937 cell lysates treated with pervanadate. IHC-P: Human lymph node tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP573-4
Isotype

IgG
Research areas

Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

All lanes : Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338) at 1/2000 dilution

Lane 1 : untreated U937 cell lysates
Lane 2 : U937 cell lysates treated with pervanadate.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit, HRP labeled, at 1/2000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Beta tubulin shows equal lysate loading amount per lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

Immunohistochemical analysis of paraffin-embedded human lymph node tissue sections labeling Syk with purified ab62338 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

Immunocytochemistry/Immunofluorescence analysis of Ramos+/-pervanadate(1mM, 30min), Ramos + pervanadate(1mM, 30min) + LP labelling Syk (phospho Y323) with ab62338 at a dilution of 1:1000 dilution (2.53ug/ml). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) (1:1000) was used as the secondary antibody. The cells were co-stained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
Flow Cytometry - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

Flow Cytometry analysis of U937 (human monocyte histiocytic lymphoma) treated (red)/untreated (green) with 1mM pervanadate for 30 minutes with purified ab62338 at 1/2500 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

Dot blot analysis of Sky (pY323) peptide (Lane 1), Syk (unmodified) peptide (Lane 2), labelling Syk (pY323) with ab62338 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.
Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

All lanes : Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338) at 1/5000 dilution (Diluted with 5% NFDM /TBST)

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with 5% NFDM/TBST
Lane 2 : Ramos treated with 1mM pervanadate for 30min whole cell lysate with 5% NFDM/TBST
Lane 3 : Ramos treated with 1mM pervanadate for 30min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. with 5% NFDM/TBST

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 72 kDa
Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

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