Mouse JAM-A ELISA Kit (ab267642)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.192 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse JAM-A ELISA Kit
See all Junctional Adhesion Molecule 1/JAM-A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
8.192 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.7 95% - 119% Plasma 109 95% - 120% Cell culture media 118.9 112% - 122% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse JAM-A ELISA Kit is designed for the quantitative determination of JAM-A in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse JAM-A coated on a 96-well plate. Standards and samples are pipetted into the wells and JAM-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse JAM-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of JAM-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpah, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse JAM-A coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse JAM-A Detection Antibody 2 vials Mouse JAM-A Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Alternative names
- CD 321
- CD321
- CD321 antigen
see all -
Database links
- Entrez Gene: 16456 Mouse
- SwissProt: O88792 Mouse
- Unigene: 294882 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.