Anti-DOK1 (phospho Y398) antibody (ab75741)
Key features and details
- Rabbit polyclonal to DOK1 (phospho Y398)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK1 (phospho Y398) antibody
See all DOK1 primary antibodies -
Description
Rabbit polyclonal to DOK1 (phospho Y398) -
Host species
Rabbit -
Specificity
ab75741 detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).
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Positive control
- Human breast carcinoma tissue. Extracts from K562 cells, starved.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75741 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y398) antibody (ab75741)ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-DOK1 (phospho Y398) antibody (ab75741)All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution
Lane 1 : K562 cell extracts, unstarved
Lane 2 : K562 cell extracts, starved
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
