Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (ab218257)
Overview
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Product name
Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel -
Product overview
Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel ab218257 contains recombinant rabbit monoclonal antibodies against MAP2, NeuN, Neurofilament heavy polypeptide, PSD95, and Synaptophysin.
Since mature neurons are no longer able to divide their purpose focuses on receiving, processing, and transferring information in the central and peripheral nervous systems.
The antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 packs ab183830 - Anti-MAP2 antibody [EPR19691] 2 x 10µl ab177487 - Anti-NeuN antibody [EPR12763] 2 x 10µl ab207176 - Anti-Neurofilament heavy polypeptide antibody [EPR20020] 2 x 10µl ab76115 - Anti-PSD95 antibody [EP2652Y] 2 x 10µl ab32127 - Anti-Synaptophysin antibody [YE269] 2 x 10µl -
Research areas
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Cellular localization
Synaptophysin: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. PSD95: Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. MAP2: Cytoplasm, cytoskeleton. NeuN: Nucleus. Cytoplasm.
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
ab177487 staining NeuN in Mouse embryonic day 15 brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/500 in PBS + 1% serum + 0.1% Triton X-100) for 16 hours at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit IgG (H+L) polyclonal (1/700) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] (ab183830)](https://www.abcam.com/ps/products/218/ab218257/Images/ab218257-274368-mature-neuron-marker-panel-immunohistochemistry-frozen-sections.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] (ab183830)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse Cortex tissue labeling MAP2 with ab183830 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on neurons of mouse Cortex is observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)](https://www.abcam.com/ps/products/218/ab218257/Images/ab218257-274367-mature-neuron-marker-panel-immunocytochemistry-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.
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![Western blot - Anti-PSD95 antibody [EP2652Y] (ab76115)](https://www.abcam.com/ps/products/218/ab218257/Images/ab218257-274366-mature-neuron-marker-panel-western-blot.jpg)
Western blot - Anti-PSD95 antibody [EP2652Y] (ab76115) This image is courtesy of an anonymous AbreviewAll lanes : Anti-PSD95 antibody [EP2652Y] (ab76115) at 1/2000 dilution
Lane 1 : Rat brain homogenate with nuclear fraction removed at 10 µg
Lane 2 : Rat brain homogenate with nuclear fraction removed at 20 µg
Lane 3 : Rat brain homogenate with nuclear fraction removed at 30 µg
Lane 4 : Rat brain homogenate with nuclear fraction removed at 40 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/1000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)Exposure time : 5 minutes
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Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (ab218257)
