Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (ab226481)
Overview
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Product name
Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel -
Product overview
Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (ab226481) is an astrocyte marker panel containing 4 recombinant rabbit monoclonal antibodies and 1 Anti-Rabbit IgG (HRP) secondary antibody: Anti-GFAP antibody, Anti-ALDH1L1 antibody, Anti-EAAT1 antibody, Anti-EEAT2 antibody, and Goat Anti-Rabbit IgG (H&L) (HRP).
Astrocytes are star-shaped glial cells and serve a wide variety of functions in the central nervous system, which are vital for brain development, physiology and pathology.
The antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 packs ab177463 - Anti-ALDH1L1 antibody [EPR12743(B)] 1 x 10µl ab181036 - Anti-EAAT1 antibody [EPR12686] 1 x 10µl ab205248 - Anti-EAAT2 antibody [EPR19798] 1 x 10µl ab68428 - Anti-GFAP antibody [EPR1034Y] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H&L (HRP) 1 x 100µg -
Research areas
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Cellular localization
GFAP: Cytoplasm. Associated with intermediate filaments. EAAT1: Membrane. EAAT2: Membrane. ALDH1L1: Cytoplasm.
Images
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Immunocytochemistry/Immunofluorescence analysis of mouse cerebellum tissue lysate labelling GFAP with ab68428 at 1/100. The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Antigen retrieval was performed by heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Nuclei were counterstained with DAPI (blue). PBS was used instead of the primary antibody as negative control.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854]. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution.
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