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Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (ab254017)

Overview

  • Product name

    Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human

  • Species reactivity

    Reacts with: Human

  • Product overview

    Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human ab254017 contains multiple trial-sized versions of anti-human antibody clones against CD103, LY75 / DEC-205, TLR7, DC-SIGN + DC-SIGNR, specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies against human CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR. They are provided as a sampler panel to allow you to easily evaluate each in your required applications. 


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [EPR4166(2)] to CD103 (20 µL) ab129202


    - Rabbit monoclonal [EPR5233] to LY75/DEC-205 (20 µL) ab124897


    - Rabbit monoclonal [EPR2088(2)] to TLR7 (20 µL) ab124928


    - Rabbit monoclonal [EPR22395-72] to DC-SIGN + DC-SIGNR (20 µL) ab245115

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human skin (PMID: 11859097; PMID: 11859097) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]
    Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) seconday antibody (green) at 1/1000 dilution.

    Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1(PMID: 15070901; PMID: 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)(red) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)]

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233]

    Paraffin-embedded, formalin-fixed staining of LY75/DEC-205 with ab124897 in Human Tonsil tissue by immunohistochemistochemical analysis at 1/250 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)]

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with purified ab129202 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody (inset).

    Counterstained with hematoxylin.

  • Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (ab254017)
    Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (ab254017)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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