Bovine IL-1 alpha ELISA Kit (ab273196)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine IL-1 alpha ELISA Kit
See all IL-1 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
16.38 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.68 86% - 104% Plasma 89.82 84% - 101% Cell culture media 112.6 108% - 119% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Bovine IL-1 alpha ELISA Kit (ab273196) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine IL-1 alpha coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- bovine IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Bovine IL-1 alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine IL-1 alpha Antibody 2 vials Bovine IL-1 alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Alternative names
- BAF
- FAF
- Hematopoietin 1
see all -
Database links
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
