Human BCA1 ELISA Kit (CXCL13) (ab179881)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human BCA1 ELISA Kit (CXCL13)
See all BCA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 3% Inter-assay Sample n Mean SD CV% Overall 3 4.1% -
Sample type
Cell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95.1 92.4% - 97.7% Cell culture media 103.7 98.9% - 107.5% Hep Plasma 106 103.8% - 107.9% EDTA Plasma 101.7 99.2% - 107% Cit plasma 101.2 96.7% - 111.3% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BCA1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued when inventory is depleted. The new version is available as ab269370.
Human BCA1 ELISA kit (ab179881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BCA1 protein in in plasma, serum and culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human BCA1 with 3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Human B-lymphocyte chemoattractant/B Cell-attracting Chemokine 1 (BCA1/CXCL13) is a member of the CXC chemokine superfamily. BCA1 (CXCL13) is produced by stromal cells from the liver, spleen, lymph nodes and the gut. It binds to the CXCR5 receptor in lymphoid follicles controlling the homing signal of B cells and subsets of T cells to the lymphoid nodes, compartmentalizing lymphocytes and antigen presenting cells within the follicles of lymphoid tissues. BCA-1 (CXCL13) is also required for the embryonic development of most of lymph nodes and Peyer’s patches. CXCL13-/- as well as CXCR5-/- mice lack secondary lymphoid structures and show a disrupted splenic architecture.
Furthermore, BCA1 (CXCL13) is considered an essential chemokine critical for the formation of ectopic lymphoid tissues within the central nervous system (CNS) and for the chemoattraction of B cells to the CNS in multiple neurological diseases. Elevated levels of the protein have been found in the cerebrospinal fluid of patients with Multiple Sclerosis, Neuroborreliosis, Neurosyphilis and Neuromyelitis optica. Elevated levels of BCA-1(CXCL13) in serum have also been correlated with HIV non Hodgking B cell lymphoma, Post-transplant lymphoproliferative disease and childhood-onset Lupus disease.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BCA-1(CXCL13) Capture Antibody 1 x 600µl 10X Human BCA-1(CXCL13) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BCA-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Relevance
B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. -
Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- SwissProt: O43927 Human
Images
-
Other - Human BCA1 ELISA Kit (CXCL13) (ab179881)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of BCA-1 (CXCL13) standard curve.Background-subtracted data values (mean +/- SD) are graphed.
-
BCA-1 (CXCL13) signal on stimulated and non stimulated media supernatants from THP-1 cells.THP-1 cells were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 8 hours with 1 µg/mL of Human IFNγ recombinant protein followed with 1 µg/mL of LPS or vehicle overnight at 37⁰C. The concentrations of BCA1 (CXCL13) were interpolated from the calibration curve and corrected for sample dilution. The mean BCA1 (CXCL13) concentration was determined to be 8.4 pg/mL in IFNγ only treated cells and 241 pg/mL in IFNγ/LPS treated cells.
-
BCA-1 (CXCL13) signal on stimulated and non stimulated media supernatants from PBMCs.Human PBMC were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of BCA1 (CXCL13) were interpolated from the calibration curve and corrected for sample dilution. The mean BCA1 (CXCL13) concentration was determined to be undetectable in unstimulated PBMC supernatants and 22.3 pg/mL in stimulated PBMCs
