Human BCA1 ELISA Kit (ab99981)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 1.37 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BCA1 ELISA Kit
See all BCA1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
1.37 pg/ml - 1000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.62 84% - 105% Serum 95.79 82% - 105% Plasma 93.68 83% - 104% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s BCA1 (BLC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human BCA1 ELISA Kit (CXCL13) (ab179881) from our SimpleStep ELISA® range.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml BCA1 Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human BCA1 2 vials Recombinant Human BCA1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. -
Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- SwissProt: O43927 Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml BCA1 Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human BCA1 2 vials Recombinant Human BCA1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. -
Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- SwissProt: O43927 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab99981.
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Typical Standard CurveRepresentative Standard Curve using ab99981.
