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Human BCA1 ELISA Kit (ab99981)

Key features and details

  • Sensitivity: 1.5 pg/ml
  • Range: 1.37 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human BCA1 ELISA Kit
    See all BCA1 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

  • Range

    1.37 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.62 84% - 105%
    Serum 95.79 82% - 105%
    Plasma 93.68 83% - 104%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s BCA1 (BLC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human BCA1 ELISA Kit (CXCL13) (ab179881) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    BCA1 Microplate (12 x 8 wells) 1 unit
    Biotinylated anti-Human BCA1 2 vials
    Recombinant Human BCA1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.

  • Alternative names

    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell chemoattractant
    • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
    • B lymphocyte chemoattractant
    • BCA 1
    • BLC
    • BLR1L
    • C X C motif chemokine 13
    • Chemokine (C X C motif) ligand 13
    • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
    • Chemokine CXC motif ligand 13
    • CXC chemokine BLC
    • CXCL13
    • SCYB13
    • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    see all

  • Database links

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      200X HRP-Streptavidin Concentrate 1 x 200µl
      20X Wash Buffer Concentrate 1 x 25ml
      5X Assay Diluent B 1 x 15ml
      Assay Diluent A 1 x 30ml
      BCA1 Microplate (12 x 8 wells) 1 unit
      Biotinylated anti-Human BCA1 2 vials
      Recombinant Human BCA1 Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml

    • Research areas

    • Relevance

      B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.

    • Alternative names

      • ANGIE
      • ANGIE2
      • B cell attracting chemokine 1
      • B cell chemoattractant
      • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
      • B lymphocyte chemoattractant
      • BCA 1
      • BLC
      • BLR1L
      • C X C motif chemokine 13
      • Chemokine (C X C motif) ligand 13
      • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
      • Chemokine CXC motif ligand 13
      • CXC chemokine BLC
      • CXCL13
      • SCYB13
      • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
      • Small inducible cytokine B13
      • Small inducible cytokine subfamily B, member 13
      see all

    • Database links

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99981.

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99981.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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