Anti-ULK3 antibody (ab240904)
Key features and details
- Rabbit polyclonal to ULK3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ULK3 antibody
See all ULK3 primary antibodies -
Description
Rabbit polyclonal to ULK3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ULK3 aa 166-177.
Database link: Q6PHR2 -
Positive control
- WB: HeLa and A549 whole cell lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ULK3 antibody (ab240904)
4% Formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for ULK3 (green) using ab240904 at 1/100 dilution for 60 minutes at RT in ICC/IF analysis. Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 min at RT was used as the secondary antibody. Phalloidin Texas Red F-Actin stain at 1/1000 dilution for 60 minutes at RT was used to detect actin. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK3 (D) Composite.
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Western blot - Anti-ULK3 antibody (ab240904)Anti-ULK3 antibody (ab240904) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 15 µg
Secondary
Anti-Rabbit IgG HRP at 1:2000 for 60 min at RT.
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 6 minutesPrimary incucbation for 16 hours at room temperature.
Block: 2% BSA and 2% Skim Milk in 1X TBST
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Western blot - Anti-ULK3 antibody (ab240904)Anti-ULK3 antibody (ab240904) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 6 minutesPrimary incubation for 1 hour at room temperature.
Block: 5% Skim Milk in 1X TBST
