Mouse Decorin ELISA Kit (ab207618)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.13 ng/ml
- Range: 0.63 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Decorin ELISA Kit
See all Decorin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.52% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.13 ng/ml -
Range
0.63 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 79 71% - 86% Serum 102 97% - 106% Cell culture media 98 97% - 98% Hep Plasma 106 97% - 110% EDTA Plasma 90 89% - 91% Cit plasma 86 84% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse Decorin ELISA Kit (ab207618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Decorin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Decorin with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Decorin is a secreted proteoglycan of the small leucine-rich proteoglycan (SLRP) family. Decorin binds to type I and type II collagen fibrils as well as to fibronectin, TGF-beta and epidermal growth factor receptor.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Decorin Capture Antibody 1 x 600µl 10X Mouse Decorin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse Decorin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Bone proteoglycan II
- CSCD
- DCN
see all -
Database links
- Entrez Gene: 13179 Mouse
- SwissProt: P28654 Mouse
- Unigene: 56769 Mouse
Images
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Other - Mouse Decorin ELISA Kit (ab207618)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Decorin standard curve in Sample Diluent NS with enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Decorin standard curve in Sample Diluent 15BS with enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Decorin in mouse serum, and plasma samples.The concentrations of Decorin were measured in duplicates, interpolated from the Decorin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, and plasma (EDTA) 25%.
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Interpolated concentrations of Decorin in mouse serum, and plasma samples.The concentrations of Decorin were measured in duplicates, interpolated from the Decorin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Decorin concentration was determined to be 12.3 ng/mL in serum, 10.7 ng/mL in plasma (citrate), 11.7 ng/mL in plasma (EDTA), and 19.2 ng/mL in plasma (heparin).
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Sandwich ELISA - Mouse Decorin ELISA Kit (ab207618)To learn more about the advantages of recombinant antibodies see here.
