Mouse Decorin Matched Antibody Pair Kit (ab215410)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Mouse Decorin Matched Antibody Pair Kit
See all Decorin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
21.71 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Decorin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Decorin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab207618. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Decorin Capture Antibody 2 x 50µg 1 x 50µg Mouse Decorin Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Decorin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Bone proteoglycan II
- CSCD
- DCN
see all -
Database links
- Entrez Gene: 13179 Mouse
- SwissProt: P28654 Mouse
- Unigene: 56769 Mouse
Images
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Mouse Decorin standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Decorin Matched Antibody Pair Kit (ab215410)To learn more about the advantages of recombinant antibodies see here.
