Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)
![Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)](https://www.abcam.com/ps/products/250/ab250509/Images/ab250509-407888-anti-apc6-antibody-epr16889-bsa-and-azide-free-western-blot.jpg "Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16889] to Apc6 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Apc6 antibody [EPR16889] - BSA and Azide free
See all Apc6 primary antibodies -
Description
Rabbit monoclonal [EPR16889] to Apc6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250509 is the carrier-free version of ab181567. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250509 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16889 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution
Lane 1 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysate at 20 µg
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Lane 4 : HT1080 (Human fibrosarcoma cells) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)](https://www.abcam.com/ps/products/250/ab250509/Images/ab250509-405905-anti-apc6-antibody-epr16889-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)](https://www.abcam.com/ps/products/250/ab250509/Images/ab250509-403500-anti-apc6-antibody-epr16889-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)](https://www.abcam.com/ps/products/250/ab250509/Images/ab250509-1-anti-apc6-antibody-epr16889-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)This data was developed using ab181567, the same antibody clone in a different buffer formulation.APC6 Protein was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181567 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181567 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)](https://www.abcam.com/ps/products/250/ab250509/Images/ab250509-5-benefits-of-recombinant-antibodies.png)
Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)