Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15828] to DSTN - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-DSTN antibody [EPR15828] - BSA and Azide free
    See all DSTN primary antibodies

  • Description

    Rabbit monoclonal [EPR15828] to DSTN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251129 is the carrier-free version of ab192262. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251129 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    Western blot - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    All lanes : Anti-DSTN antibody [EPR15828] (ab192262) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa



    This data was developed using ab192262, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    Immunocytochemistry - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    This data was developed using ab192262, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling DSTN using ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    This data was developed using ab192262, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue sections labeling DSTN using ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    Immunoprecipitation - Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    This data was developed using ab192262, the same antibody clone in a different buffer formulation.Lysate from A549 cells was immunoprecipitated with ab192262 at a 1/30 dilution. For the subsequent blot, ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
  • Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)
    Anti-DSTN antibody [EPR15828] - BSA and Azide free (ab251129)

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