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Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)

Overview

  • Product name

    Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human

  • Species reactivity

    Reacts with: Human

  • Product overview

    Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human ab254013 contains multiple trial-sized versions of anti-human antibody clones against CD11b, CD68, CD163, CD14, CD16, specifically selected for high performance in various applications. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD11b, CD68, CD163, CD14, CD16. They are provided as a sampler panel to allow you to easily evaluate each in your required applications. 


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [EP1345Y] to CD11b (20 µL) ab52478


    - Rabbit monoclonal [EPR20545] to CD68 (20 µL) ab213363


    - Rabbit monoclonal [EPR19518] to CD163 (20 µL) ab182422


    - Rabbit monoclonal [SP192] to CD14 (20 µL) ab183322


    - Rabbit monoclonal [SP175] to CD16 (20 µL) ab183354

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y]

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue sections labeling CD11b with purified ab52478 at 1:1000 dilution (0.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Hematoxylin was used as a counterstain.

    Negative control: PBS instead of the primary antibody (inset).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y]
    Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y]

    Unpurified ab52478 staining CD11b in the THP-1 (Human monocytic leukemia cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545]

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling CD68 with ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human cervical carcinoma is observed (PMID: 12118106). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545]
    Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545]

    Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling CD68 with ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518]

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Kupffer cells of human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192]

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [SP175]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [SP175]

    Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD16 with ab183354 at 1/100 dilution.

  • Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
    Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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