T cell Activation Marker (CD69, CD137, CD27, TRAP/CD40L, CD134) Antibody Panel - Human (ab254024)
Overview
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Product name
T cell Activation Marker (CD69, CD137, CD27, TRAP/CD40L, CD134) Antibody Panel - Human -
Species reactivity
Reacts with: Human -
Product overview
T cell Activation Marker (CD69, CD137, CD27, TRAP/CD40L, CD134) Antibody Panel - Human ab254024 contains multiple trial-sized versions of anti-human antibody clones against CD69, CD137, CD27, TRAP/CD40L, CD134, specifically selected for high performance in various applications. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD69, CD137, CD27, TRAP/CD40L, CD134 They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR21814] to CD69 (20 µL) ab233396
- Rabbit monoclonal [BLR051F] to CD137 (20 µL) ab252559
- Rabbit monoclonal [EPR8569] to CD27 (20 µL) ab131254
- Rabbit monoclonal [EP462E] to TRAP/CD40L (20 µL) ab52750
- Rabbit monoclonal [BLR042F] to CD134 / OX40L receptor (20 µL) ab243898
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab243898 - Anti-CD134 / OX40L receptor antibody [BLR042F] 2 x 10µl ab252559 - Anti-CD137 antibody [BLR051F] 2 x 10µl ab131254 - Anti-CD27 antibody [EPR8569] 2 x 10µl ab233396 - Anti-CD69 antibody [EPR21814] 2 x 10µl ab52750 - Anti-TRAP/CD40L antibody [EP462E] 2 x 10µl -
Research areas
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Cellular localization
CD27: Membrane. CD69: Membrane. CD137: Membrane. CD134 / OX40L receptor: Membrane. TRAP/CD40L: Secreted and Cell membrane. -
Database links
- Entrez Gene: 3604 Human
- Entrez Gene: 7293 Human
- Entrez Gene: 939 Human
- Entrez Gene: 959 Human
- Entrez Gene: 969 Human
- Omim: 107273 Human
- Omim: 186711 Human
- Omim: 300386 Human
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Images
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Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CD134 / OX40L using ab243898 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD27 with purified ab131254 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab252559 at a 1/200 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD69 with ab233396 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in lymphoid cells of human tonsil (PMID: 2807372) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -