Antibodies, Proteins, Kits and Reagents for Life Science

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PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

Product name

PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106)
See all PD1 kits
Species reactivity

Reacts with: Human
Product overview

PD1 Antibody Panel ab252192 contains multiple trial-sized versions of anti-human antibody clones against PD1, specifically selected for high performance in IHC. This panel contains 5 recombinant rabbit and mouse monoclonal antibodies against human PD1. They are provided in trial sizes to allow you to easily evaluate which is the best PD1 antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL20] to PD1 (20 µL) ab237728

- Rabbit monoclonal [EPR4877(2)] to PD1 (20 µL) ab137132

- Mouse monoclonal [NAT105] to PD1 (20 µL) ab52587

- Rabbit monoclonal [NAT105-EPR21106] to PD1 - Chimeric (20 µL) ab216352

- Rabbit monoclonal [SP269] to PD1 (20 µL) ab227681
Notes

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass Cytometry^TM. Please refer to the ‘Associated products’ section below.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Storage instructions

Store at -20°C. Please refer to protocols.

Components	1 kit
ab237728 - Anti-PD1 antibody [CAL20]	2 x 10µl
ab137132 - Anti-PD1 antibody [EPR4877(2)]	2 x 10µl
ab52587 - Anti-PD1 antibody [NAT105]	1 x 20µl
ab216352 - Anti-PD1 antibody [NAT105-EPR21106] - Chimeric	2 x 10µl
ab227681 - Anti-PD1 antibody [SP269]	2 x 10µl

Research areas
- Cancer
- Cell Death
- Apoptosis
- Other
Function

Possible cell death inducer, in association with other factors.
Involvement in disease

Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Developmental stage

Induced at programmed cell death.
Cellular localization

Membrane.
Target information above from: UniProt accession Q15116 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- CD279
- CD279 antigen
- hPD 1
- hPD l
- hPD-1
- hSLE1
- PD 1
- PD-1
- PD1
- PDCD 1
- PDCD1
- PDCD1_HUMAN
- Programmed cell death 1
- Programmed cell death 1 protein
- Programmed cell death protein 1
- Protein PD 1
- Protein PD-1
- SLEB2
- Systemic lupus erythematosus susceptibility 2
see all
Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [SP269]

Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using ab227681 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105-EPR21106]

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab216352 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on T cells of human tonsil germinal center (PMID: 20087161; PMID: 17640856) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Perform antigen retrieval using Universal HIER antigen retrieval reagents (10X) (ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105]

IHC image of PD1 staining in normal human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52587 at 5 µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)]]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.

Image scanned with Vectra 3.0 and analyzed via Inform software.

This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [CAL20]

Formalin-fixed, paraffin-embedded human lymph node tissue stained for PD1 using ab237728 at 0.125 µg/ml dilution in immunohistochemical analysis.
PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

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