Astana Biomed Group, an authorized Abcam distributor in Central Asia

CD163 Antibody Panel (EPR14643-36, EPR19518, EPR14643) (ab252200)

Overview

  • Product name

    CD163 Antibody Panel (EPR14643-36, EPR19518, EPR14643)
    See all CD163 kits

  • Species reactivity

    Reacts with: Human

  • Product overview

    CD163 Antibody Panel ab252200 contains multiple trial-sized versions of anti-human antibody clones against CD163, specifically selected for high performance in IHC. This panel contains 3 recombinant rabbit monoclonal antibodies against human CD163. They are provided in trial sizes to allow you to easily evaluate which is the best CD163 antibody for your IHC research assay needs.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [EPR14643] to CD163 - C-terminal (20 µL) ab188571


    - Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal (20 µL) ab189915


    - Rabbit monoclonal [EPR19518] to CD163 (20 µL) ab182422

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    ab188571 - Anti-CD163 antibody [EPR14643] - C-terminal 2 x 10µl
    ab189915 - Anti-CD163 antibody [EPR14643-36] - C-terminal 2 x 10µl
    ab182422 - Anti-CD163 antibody [EPR19518] 2 x 10µl

  • Research areas

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

  • Tissue specificity

    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

  • Sequence similarities

    Contains 9 SRCR domains.

  • Domain

    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.

  • Post-translational
    modifications

    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.

  • Cellular localization

    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt

  • Alternative names

    • C163A_HUMAN
    • CD 163
    • CD163
    • CD163 antigen
    • CD163 molecule
    • Hemoglobin scavenger receptor
    • M130
    • M130 antigen precursor
    • Macrophage associated antigen
    • MM130
    • OTTHUMP00000238617
    • OTTHUMP00000238618
    • OTTHUMP00000238619
    • OTTHUMP00000238620
    • SCARI1
    • Scavenger receptor cysteine rich type 1 protein M130
    • sCD163
    • Soluble CD163
    see all

  • Database links

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR19518]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR19518]

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse spleen is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR14643-36]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR14643-36]

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR14643]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 [EPR14643]

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CD163 with ab188571 at 1/500 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    • CD163 Antibody Panel (EPR14643-36, EPR19518, EPR14643) (ab252200)
      CD163 Antibody Panel (EPR14643-36, EPR19518, EPR14643) (ab252200)

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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