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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)

Price and availability

241 228 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR576(2)Y] to Bcr (phospho Y177)
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Bcr (phospho Y177) antibody [EPR576(2)Y]
    See all Bcr primary antibodies
  • Description

    Rabbit monoclonal [EPR576(2)Y] to Bcr (phospho Y177)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Bcr (phospho Y177). The exact sequence is proprietary.

  • Positive control

    • 3T3 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR576(2)Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other

Images

  • Western blot - Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)
    Western blot - Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)
    All lanes : Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216) at 1/1000 dilution

    Lane 1 : 3T3 cell lysate, membrane un-treated
    Lane 2 : 3T3 cell lysate, membrane treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 143 kDa
    Observed band size: 153 kDa
    why is the actual band size different from the predicted?

  • Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)
    Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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