Anti-Bcr antibody [1E11G12] (ab233709)
![Anti-Bcr antibody [1E11G12] (ab233709)](https://www.abcam.com/ps/products/233/ab233709/Images/ab233709-307043-anti-bcr-antibody-1e11g12-western-blot.jpg "Anti-Bcr antibody [1E11G12] (ab233709)")
Key features and details
- Mouse monoclonal [1E11G12] to Bcr
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Bcr antibody [1E11G12]
See all Bcr primary antibodies -
Description
Mouse monoclonal [1E11G12] to Bcr -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Bcr aa 100-300. Expressed in E.coli.
Database link: P11274 -
Positive control
- WB: Recombinant human Bcr (aa139-280) protein; Human Bcr (aa139-280)-IgG-Fc-transfected HEK-293 cell lysate; Jurkat, HeLa and Ramos cell lysates. Flow cyt: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1E11G12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Bcr antibody [1E11G12] (ab233709)Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution + Recombinant human Bcr (aa139-28) protein
Developed using the ECL technique.
Predicted band size: 143 kDa
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![Western blot - Anti-Bcr antibody [1E11G12] (ab233709)](https://www.abcam.com/ps/products/233/ab233709/Images/ab233709-307044-anti-bcr-antibody-1e11g12-western-blot.jpg)
Western blot - Anti-Bcr antibody [1E11G12] (ab233709)All lanes : Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human Bcr (aa139-280)-IgG-Fc-transfected HEK-293 cell lysate
Developed using the ECL technique.
Predicted band size: 143 kDa
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![Western blot - Anti-Bcr antibody [1E11G12] (ab233709)](https://www.abcam.com/ps/products/233/ab233709/Images/ab233709-307045-anti-bcr-antibody-1e11g12-western-blot.jpg)
Western blot - Anti-Bcr antibody [1E11G12] (ab233709)All lanes : Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 143 kDa
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![Flow Cytometry - Anti-Bcr antibody [1E11G12] (ab233709)](https://www.abcam.com/ps/products/233/ab233709/Images/ab233709-307046-anti-bcr-antibody-1e11g12-flow-cytometry.jpg)
Flow Cytometry - Anti-Bcr antibody [1E11G12] (ab233709)Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Bcr with ab233709 at 1/200 dilution (green) compared with a negative control (red).
