Anti-Bcr antibody (ab245511)
Key features and details
- Rabbit polyclonal to Bcr
- Suitable for: IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcr antibody
See all Bcr primary antibodies -
Description
Rabbit polyclonal to Bcr -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bcr aa 50-100. The exact sequence is proprietary. NP_004318.3
Database link: P11274 -
Positive control
- IP: Bcr IP in HeLa whole cell lysate. Flow Cyt (Intra): K562 cells. ICC/IF: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245511 was affinity purified using an epitope specific to Bcr immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody (ab245511)
Formalin-fixed, paraffin-embedded K562 (human chronic myelogenous leukemia cell line from bone marrow, contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene) cells stained for Bcr (Green) using ab245511 at 1/1,000 dilution. Green-fluorescent goat anti rabbit IgG - Alexa Fluor® 488 was used as the secondary antibody at a dilution of 1/1,000.
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Immunoprecipitation - Anti-Bcr antibody (ab245511)Bcr was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded). Western blot was performed on the immunoprecipitate using a different rabbit anti-Bcr primary antibody.
Lane 1: ab245511 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
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Flow Cytometry - Anti-Bcr antibody (ab245511)Flow cytometric analysis of 1 x 106 K562 ( human chronic myelogenous leukemia cell line from bone marrow) cells stained with 0.25 µg ab245511 in a 150 µl reaction (Blue). Isotype anti-KLH control (black), no antibody (red).
