SLAMF7 Antibody Panel (EPR21155, CAL7) (ab252203)
Overview
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Product name
SLAMF7 Antibody Panel (EPR21155, CAL7)
See all SLAMF7/CS1 kits -
Species reactivity
Reacts with: Human -
Product overview
SLAMF7 Antibody Panel ab252203 contains multiple trial-sized versions of anti-human antibody clones against SLAMF7, specifically selected for high performance in IHC. This panel contains 2 recombinant rabbit monoclonal antibodies against human SLAMF7. They are provided in trial sizes to allow you to easily evaluate which is the best SLAMF7 antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [CAL7] to SLAMF7 (20 µL) ab237730
- Rabbit monoclonal [EPR21155] to SLAMF7 (20 µL) ab230945
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab237730 - Anti-SLAMF7 antibody [CAL7] 2 x 10µl ab230945 - Anti-SLAMF7 antibody [EPR21155] 2 x 10µl -
Research areas
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Relevance
SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Alternative names
- 19A
- 19A24 protein
- CD 319
see all -
Database links
- Entrez Gene: 57823 Human
- Omim: 606625 Human
- SwissProt: Q9NQ25 Human
- Unigene: 517265 Human
Images
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Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling SLAMF7 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in human multiple myeloma (PMID: 26005365; PMID: 18451245) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Formalin-fixed, paraffin-embedded multiple myeloma (Lambda, left panel) and SLAMF7 Knock-in AD-293 cells (Positive control, right panel) stained for SLAMF7 using ab237730 at 0.1 µg/ml dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded OPM2 xenograft (Left panel), and NCI-N87 xenograft (Right panel) tissue stained for SLAMF7 using ab237729 at 0.1 µg/ml dilution in immunohistochemical analysis.
Negative control: NCI-N87 xenograft (Right panel).
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