All lanes : Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution

Lane 1 : Wild-type THP1 cell lysate
Lane 2 : LYZ knockout THP1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 16 kDa
Observed band size: 16 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab223503 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab223503 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab223503 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate

Predicted band size: 16 kDa

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.