Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR2994(2)] to Lysozyme
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Lysozyme antibody [EPR2994(2)]
    See all Lysozyme primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR2994(2)] to Lysozyme

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, HepG2, HL-60 whole cell lysate and human spleen tissue lysate. IHC-P: normal human tonsil tissue sections.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    Western blot - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    All lanes : HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705) at 1/1000 dilution

    Lane 1 : Wild-type THP-1 cell lysate
    Lane 2 : LYZ knockout THP-1 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    ab197705 was shown to react with Lysozyme in wild-type THP-1 cells in western blot. Loss of signal was observed when LYZ knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab197705 overnight at 4 °C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)

    IHC image of lysozyme staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197705, 1/100 dilution, for 15 mins at room temperature.

    DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

     

  • Western blot - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    Western blot - HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    All lanes : HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705) at 1/1000 dilution

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197705 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)
    HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705)

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