Mouse SLPI ELISA Kit (ALP) is designed for the quantitative determination of SLPI in cell culture supernatants, plasma and serum samples.

Δ Note: Mouse SLPI concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for mouse SLPI coated on a 96-well plate. Standards and samples are pipetted into the wells and SLPI present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SLPI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SLPI bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.

• Immunology
• Innate Immunity
• Macrophage / Inflamm.

• Immunology
• Adaptive Immunity
• B Cells
• Non-CD

• Immunology
• Adaptive Immunity
• T Cells
• Non-CD

• Cell Biology
• Proteolysis / Ubiquitin
• Protease inhibitors
• Other protease inhibitors

• Cardiovascular
• Atherosclerosis
• Vascular Inflammation
• Leukocyte recruitment
• Other