Rat CINC-3 ELISA Kit (CXCL2) (ab155463)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 7.4 pg/ml - 1800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CINC-3 ELISA Kit (CXCL2) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
7.4 pg/ml - 1800 pg/mlRecovery
Sample specific recovery Sample type Average % Range Plasma 91.87 72% - 111% Cell culture media 83.48 75% - 89% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: RatProduct overview
Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-rat CINC-3 2 vials CINC-3 Microplate (12 x 8 wells) 1 unit Recombinant Rat CINC-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- C-X-C motif chemokine 2
- CINC 3
- Cinc3
see all -
Database links
- Entrez Gene: 114105 Rat
- SwissProt: P30348 Rat
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-rat CINC-3 2 vials CINC-3 Microplate (12 x 8 wells) 1 unit Recombinant Rat CINC-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Cellular localization
Secreted -
Alternative names
- C-X-C motif chemokine 2
- CINC 3
- Cinc3
see all -
Database links
- Entrez Gene: 114105 Rat
- SwissProt: P30348 Rat
Images
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Typical Standard Curve
Representative standard curve using ab155463 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155463 - Assay Diluent C.