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Immunology Innate Immunity Macrophage / Inflamm.

Rat CINC-3 ELISA Kit (CXCL2) (ab155463)

Rat CINC-3 ELISA Kit (CXCL2) (ab155463)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Sensitivity: 2 pg/ml
  • Range: 7.4 pg/ml - 1800 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

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Overview

  • Product name

    Rat CINC-3 ELISA Kit (CXCL2)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

  • Range

    7.4 pg/ml - 1800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Plasma 91.87 72% - 111%
    Cell culture media 83.48 75% - 89%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated anti-rat CINC-3 2 vials
    CINC-3 Microplate (12 x 8 wells) 1 unit
    Recombinant Rat CINC-3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokine Rec. (CXCR)
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Cellular localization

    Secreted
  • Alternative names

    • C-X-C motif chemokine 2
    • CINC 3
    • Cinc3
    • Cxcl2
    • Cytokine-induced neutrophil chemoattractant 3
    • GRO Protein Beta
    • GRO2
    • GRO2 ONCOGENE
    • GROB
    • Macrophage inflammatory protein 2
    • MIP 2
    • Mip-2
    • Mip2
    • MIP2-ALPHA
    • MIP2A
    • Scyb2
    • SMALL INDUCIBLE CYTOKINE SUBFAMILY B, MEMBER 2
    see all
  • Database links

    • Entrez Gene: 114105 Rat
    • SwissProt: P30348 Rat

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 25ml
      5X Assay Diluent B 1 x 15ml
      600X HRP-Streptavidin Concentrate 1 x 200µl
      Assay Diluent C 1 x 30ml
      Biotinylated anti-rat CINC-3 2 vials
      CINC-3 Microplate (12 x 8 wells) 1 unit
      Recombinant Rat CINC-3 Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Innate Immunity
      • Chemokines
      • Alpha Chemokine Rec. (CXCR)
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
    • Cellular localization

      Secreted
    • Alternative names

      • C-X-C motif chemokine 2
      • CINC 3
      • Cinc3
      • Cxcl2
      • Cytokine-induced neutrophil chemoattractant 3
      • GRO Protein Beta
      • GRO2
      • GRO2 ONCOGENE
      • GROB
      • Macrophage inflammatory protein 2
      • MIP 2
      • Mip-2
      • Mip2
      • MIP2-ALPHA
      • MIP2A
      • Scyb2
      • SMALL INDUCIBLE CYTOKINE SUBFAMILY B, MEMBER 2
      see all
    • Database links

      • Entrez Gene: 114105 Rat
      • SwissProt: P30348 Rat

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155463 - Assay Diluent B.

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155463 - Assay Diluent C.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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