Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-15-PGDH antibody [EPR14332] (ab187160)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14332] to 15-PGDH
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-15-PGDH antibody [EPR14332]
See all 15-PGDH primary antibodies
Description

Rabbit monoclonal [EPR14332] to 15-PGDH
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Cco-2, SW480, LoVo and Human placenta lysates; Human stomach and Human colon tissues; SW480 and Caco-2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14332
Isotype

IgG
Research areas

Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)

All lanes : Anti-15-PGDH antibody [EPR14332] (ab187160) at 1/10000 dilution

Lane 1 : caco-2 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : LoVo cell lysate
Lane 4 : Human placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry - Anti-15-PGDH antibody [EPR14332] (ab187160)

Flow Cytometrical analysis of Caco-2 cells labeling 15-PGDH with ab187160 at 1/90 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 used for the analysis.
Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)

Immunofluorescent analysis of SW480 cells labeling 15-PGDH with ab187160 at 1/250 and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling 15-PGDH with ab187160 at 1/4000 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)

Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling 15-PGDH with ab187160 at 1/4000 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-15-PGDH antibody [EPR14332] (ab187160)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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