Anti-15-PGDH antibody (ab96732)
Key features and details
- Rabbit polyclonal to 15-PGDH
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-15-PGDH antibody
See all 15-PGDH primary antibodies -
Description
Rabbit polyclonal to 15-PGDH -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to 15-PGDH aa 255-266 (C terminal).
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Positive control
- 293T cell lysate, A431 cell lysate, HeLaS3 cell lysate, HepG2 whole cell lysate (ab7900), Molt-4 cell lysate, Raji cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-15-PGDH antibody (ab96732)Anti-15-PGDH antibody (ab96732) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody (ab96732)ab96732, at 1/500 dilution, staining 15-PGDH in Human MDA-MB-468 xenograft by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody (ab96732)ab96732 staining 15-PGDH in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in 15-PGDH expression correlates with increased concentration of diclofenac sodium salt as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody (ab96732)ab96732, at 1/200 dilution, staining 15-PGDH by immunofluorescence analysis of paraformaldehyde-fixed HeLa cells.
