Recombinant human Cytosolic Phospholipase A2 protein (ab198469)
Key features and details
- Expression system: Baculovirus
- Purity: >= 87% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cytosolic Phospholipase A2 protein
See all Cytosolic Phospholipase A2 proteins and peptides -
Biological activity
Specific Activity: 57 pmol/min/µg.
Assay Conditions: PLA reaction was done using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine
substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng ab198469. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature. -
Purity
>= 87 % SDS-PAGE. -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFI STTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDE TLGTATFTVSSMKVGEKKE VPFIFNQVTEMVLEMSLEVCSCPDLRFSM ALCDQEKTFRQQRKEHIRESMKKLLGPKN SEGLHSARDVPVVAILGSG GGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYM STLYSHPDFP EKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTD IFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELM FADWVEFSPYE IGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLM GVWGSAFSILFNRVLGVSGSQ SRGSTMEEELENITTKHIVSNDSSDSD DESHEPKGTENEDAGSDYQSDNQASWIHRMI MALVSDSALFNTREGRA GKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVAD PDEFERI YEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSS PPFK ELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEK DCPTIIHFVLANINFR KYKAPGVPRETEEEKEIADFDIFDDPESPFST FNFQYPNQAFKRLHDLMHFNTLNNIDVI KEAMVESIEYRRQNPSRCSV SLSNVEARRFFNKEFLSKPKADYKDDDDKHHHHHH -
Predicted molecular weight
87 kDa including tags -
Amino acids
1 to 749 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_024420
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Cytosolic Phospholipase A2 protein (ab198469)
4-20% SDS PAGE analysis of 0.9 μg ab198469 stained with Coomassie.
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Functional Studies - Recombinant human Cytosolic Phospholipase A2 protein (ab198469)Specific activity analysis of ab198469.