Anti-beta Catenin antibody - ChIP Grade (ab227499)
Key features and details
- Rabbit polyclonal to beta Catenin - ChIP Grade
- Suitable for: WB, IP, IHC-P, Flow Cyt, ChIP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-beta Catenin antibody - ChIP Grade
See all beta Catenin primary antibodies -
Description
Rabbit polyclonal to beta Catenin - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanFlow Cyt HumanICC/IF HumanIHC-P MouseRatHumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment within Human beta Catenin (N terminal). The exact sequence is proprietary.
Database link: P35222 -
Positive control
- IHC-P: Mouse colon, skin, intestine, duodenum and urinary bladder tissues; Human esophagus and cervix tissues; Rat colon and duodenum tissues. ICC/IF: A431, HeLa and HCT 116 cells. WB: Mouse brain lysate; PC-12, A549, NCI-H1299 and HCT 116 and HeLa whole cell extracts. IP: HeLa whole cell extract. Flow Cyt: HeLa cells. ChIP: HCT 116 chromatin extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Catenin antibody - ChIP Grade (ab227499)Anti-beta Catenin antibody - ChIP Grade (ab227499) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse colon tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis. Counterstain: Alpha-tubulin was labeled with an anti-alpha tubulin antibody at 1/500 dilution. Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse colon tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis. Counterstain: Alpha-tubulin was labeled with an anti-alpha tubulin antibody at 1/500 dilution. Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse skin tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse colon tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded human esophagus tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded human cervix tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for beta Catenin (green) using ab227499 at 1/500 dilution in ICC/IF. Counterstain: Alpha-tubulin filaments were labeled with an anti-alpha tubulin antibody at 1/2000 dilution (red).
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Western blot - Anti-beta Catenin antibody - ChIP Grade (ab227499)Anti-beta Catenin antibody - ChIP Grade (ab227499) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-beta Catenin antibody - ChIP Grade (ab227499)beta Catenin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab227499. Western blot was performed from the immunoprecipitate using ab227499. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG instead of ab227499 in HeLa whole cell extract.
Lane 3: ab227499 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse intestine tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse duodenum tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded rat colon tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded rat duodenum tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse urinary bladder tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody - ChIP Grade (ab227499)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Catenin (green) using ab227499 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody - ChIP Grade (ab227499)Paraffin-embedded mouse duodenum tissue stained for beta Catenin using ab227499 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-beta Catenin antibody - ChIP Grade (ab227499)All lanes : Anti-beta Catenin antibody - ChIP Grade (ab227499) at 1/10000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Flow Cytometry - Anti-beta Catenin antibody - ChIP Grade (ab227499)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling beta Catenin with ab227499 at 1/50 dilution (green) compared with an unlabeled sample used as a control (blue). A Dylight® 488-conjugated secondary antibody was used.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody - ChIP Grade (ab227499)4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for beta Catenin (green) using ab227499 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
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ChIP - Anti-beta Catenin antibody - ChIP Grade (ab227499)Cross-linked ChIP was performed with HCT 116 (human colorectal carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab227499 antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.
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Western blot - Anti-beta Catenin antibody - ChIP Grade (ab227499)Lanes 1-2 : Anti-beta Catenin antibody - ChIP Grade (ab227499) at 1/1000 dilution
Lane 3 : Anti-beta Catenin antibody - ChIP Grade (ab227499) at 1000 cells
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lanes 2-3 : beta Catenin shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
