HRP Anti-beta Catenin antibody [E247] (ab194120)
![HRP Anti-beta Catenin antibody [E247] (ab194120)](https://www.abcam.com/ps/products/194/ab194120/Images/ab194120-307386-ab194120HRPKOWBJRIZ.jpg "HRP Anti-beta Catenin antibody [E247] (ab194120)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E247] to beta Catenin
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-beta Catenin antibody [E247]
See all beta Catenin primary antibodies -
Description
HRP Rabbit monoclonal [E247] to beta Catenin -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U20S cell lysate. IHC-P: FFPE human colon adeoncarcinoma tissue sections.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E247 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-beta Catenin antibody [E247] (ab194120)All lanes : HRP Anti-beta Catenin antibody [E247] (ab194120) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CTNNB1 (beta Catenin) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesab194120 was shown to specifically react with beta Catenin in wild-type HAP1 cells as signal was lost in CTNNB1 (beta Catenin) knockout cells. Wild-type and CTNNB1 (beta Catenin) knockout samples were subjected to SDS-PAGE. Ab194120 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta Catenin antibody [E247] (ab194120)](https://www.abcam.com/ps/products/194/ab194120/Images/ab194120-234176-ab194120ap2115933hucolon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta Catenin antibody [E247] (ab194120)IHC image of beta Catenin staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab194120 at a working dilution of 1/500. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-beta Catenin antibody [E247] (ab194120)](https://www.abcam.com/ps/products/194/ab194120/Images/ab194120-232712-WBAP21159331.jpg)
Western blot - HRP Anti-beta Catenin antibody [E247] (ab194120)HRP Anti-beta Catenin antibody [E247] (ab194120) at 1/5000 dilution + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194120 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-beta Catenin antibody [E247] (ab194120)](https://www.abcam.com/ps/products/194/ab194120/Images/ab194120-4-benefits-of-recombinant-antibodies.png)
HRP Anti-beta Catenin antibody [E247] (ab194120)
