Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Catenin with purified ab224803 at 1/10 (9.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Flow cytometry analysis of C6 (rat glial tumor glial cell) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the rat pancreas, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Catenin with purified ab224803 at 1/10 (9.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the mouse pancreas, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous with nuclear staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224803 for 10 mins at room temperature.

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Flow cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling beta Catenin with purified ab224803 at 1/200 dilution (0.495 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This image was generated using ab224803, the same clone, but with a different buffer formulation.

Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cell line labeling beta Catenin with ab224803 at 1/400 dilution (green) compared to a negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human prostate tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human ovary adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human ovary tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human lung squamous carcinoma tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human lung tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human liver tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human kidney tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human colon tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human stomach tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human bladder transitional carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human bladder tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human breast tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).