Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-beta Catenin (phospho S37) antibody [EP742(2)Y]
See all beta Catenin primary antibodies -
Description
Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37) -
Host species
Rabbit -
Specificity
ab75777 detects beta Catenin phosphorylated on serine 37. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human beta Catenin. The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding Serine 33 and 37 of human beta Catenin
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Positive control
- 293T cell lysates, untreated or treated with calyculin A.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP742(2)Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/5000 dilution
Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates
Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates treated with MG-132.
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates treated with MG-132 then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
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Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Beta catenin (pS33+pS37) phospho peptide
Lane 2: Beta catenin (pS33) phospho peptide
Lane 3: Beta catenin (pS37) phospho peptide
Lane 4: Beta catenin non-phospho peptide -
All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/500 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
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