Anti-beta Catenin antibody [15B8] (ab6301)
![Anti-beta Catenin antibody [15B8] (ab6301)](https://www.abcam.com/ps/products/6/ab6301/Images/ab6301-284316-anti-beta-catenin-antibody-15b8-western-blot.jpg "Anti-beta Catenin antibody [15B8] (ab6301)")
Key features and details
- Mouse monoclonal [15B8] to beta Catenin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-beta Catenin antibody [15B8]
See all beta Catenin primary antibodies -
Description
Mouse monoclonal [15B8] to beta Catenin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant full length protein corresponding to Chicken beta Catenin.
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Positive control
- WB: Hap1, HeLa, A43, HEK293, Caco2, NIH3T3 and PC12 cell lysates.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
15B8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-beta Catenin antibody [15B8] (ab6301)All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CTNNB1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 85 kDaLanes 1 - 3: Merged signal (red and green). Green - ab6301 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab6301 was shown to specifically react with beta-Catenin in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and beta Catenin knockout samples were subjected to SDS-PAGE. Ab6301 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-beta Catenin antibody [15B8] (ab6301)](https://www.abcam.com/ps/products/6/ab6301/Images/ab6301-12207-anti-beta-catenin-antibody-15b8-western-blot.jpg)
Western blot - Anti-beta Catenin antibody [15B8] (ab6301)All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab6301 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
