Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)
![Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)](https://www.abcam.com/ps/products/233/ab233769/Images/ab233769-343232-ab19350339377ICCAP439715901.jpg "Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)")
Key features and details
- Mouse monoclonal [6A9] to P cadherin - BSA and Azide free
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-P cadherin antibody [6A9] - BSA and Azide free
See all P cadherin primary antibodies -
Description
Mouse monoclonal [6A9] to P cadherin - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Full length protein. Proteins removed from A431 cell membranes by trypsinization.
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Positive control
- ICC/IF: A431 cells.
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General notes
ab233769 is a PBS-only buffer format of ab19350.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6A9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)
ab19350 staining P-Cadherin in A431 cells (top panel) and MCF7 (negative cell line - bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab19350).
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![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)](https://www.abcam.com/ps/products/233/ab233769/Images/ab233769-343233-ab19350319891ICCAP439715901.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (ab233769)ab19350 staining P-Cadherin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab19350).
