Anti-Desmoglein 2/DSG2 antibody (ab96761)
Key features and details
- Rabbit polyclonal to Desmoglein 2/DSG2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmoglein 2/DSG2 antibody
See all Desmoglein 2/DSG2 primary antibodies -
Description
Rabbit polyclonal to Desmoglein 2/DSG2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Desmoglein 2/DSG2 aa 1-176.
Database link: NP_001934 -
Positive control
- WB: 293T, H1299 and MOLT4 whole cell lysates; HepG2 cell lysate; HeLa cell lysate. IHC-P: DU145 xenograft ICC/IF: A431 cells.
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General notes
This product was previously labelled as Desmoglein 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded mouse skin labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0) 15 minutes.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 30 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 122 kDa
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All lanes : Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS Page -
Immunohistochemical analysis of Desmoglein 2/DSG2 in paraffin embedded DU145 (Human) xenograft, using ab96761 at a 1/100 dilution.