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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins

Anti-E Cadherin antibody [EPR699] (ab133597)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-E Cadherin antibody [EPR699] (ab133597)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR699] to E Cadherin
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-E Cadherin antibody [EPR699]
    See all E Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR699] to E Cadherin
  • Host species

    Rabbit
  • Specificity

    E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human E Cadherin aa 150-250.
    Database link: P12830

  • Positive control

    • WB: A431, MCF7, Caco-2, HeLa whole cell lysate (ab150035).
  • General notes

    Mouse, Rat, We have preliminary internal testing data sugesting that this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR699
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Images

  • Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Anti-E Cadherin antibody [EPR699] (ab133597) at 1/1000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 97 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
    Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
    Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    All lanes : PC-3 (Human prostate adenocarcinoma epithelial cell) Whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 97 kDa



    Exposure time: 3 minutes for Ab40772 and Ab133597, 32 seconds for GAPDH

    Blocking and diluting buffer: 5% NFDM/TBST

    Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456

    This image was produced using unpurified antibody.

  • Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
    Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
    Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    All lanes : HT-29 (Human colorectal adenocarcinoma epithelial cell). Whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Exposure time: 1 second for Ab40772, 3 minutes for Ab133597, 32 seconds for GAPDH.

    Blocking and diluting buffer: 5% NFDM/TBST

    Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456

    This image was produced using unpurified antibody.

  • Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    Western blot - Anti-E Cadherin antibody [EPR699] (ab133597)
    All lanes : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/1000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
    Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 97 kDa



    This image was produced using unpurified antibody.

  • Anti-E Cadherin antibody [EPR699] (ab133597)
    Anti-E Cadherin antibody [EPR699] (ab133597)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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