Anti-E Cadherin antibody [EPR699] (ab133597)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR699] to E Cadherin
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-E Cadherin antibody [EPR699]
See all E Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR699] to E Cadherin -
Host species
Rabbit -
Specificity
E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Human E Cadherin aa 150-250.
Database link: P12830 -
Positive control
- WB: A431, MCF7, Caco-2, HeLa whole cell lysate (ab150035).
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General notes
Mouse, Rat, We have preliminary internal testing data sugesting that this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR699 -
Isotype
IgG -
Research areas
Images
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Anti-E Cadherin antibody [EPR699] (ab133597) at 1/1000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
All lanes : PC-3 (Human prostate adenocarcinoma epithelial cell) Whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 97 kDaExposure time: 3 minutes for Ab40772 and Ab133597, 32 seconds for GAPDH
Blocking and diluting buffer: 5% NFDM/TBST
Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456
This image was produced using unpurified antibody.
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Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
All lanes : HT-29 (Human colorectal adenocarcinoma epithelial cell). Whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Exposure time: 1 second for Ab40772, 3 minutes for Ab133597, 32 seconds for GAPDH.
Blocking and diluting buffer: 5% NFDM/TBST
Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456
This image was produced using unpurified antibody.
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All lanes : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDaThis image was produced using unpurified antibody.
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